High purity enzymes and buffers. Optimized for the highest activiz
Enzymes
Taq DNA Polymerase
Features
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Purity: < 98% confirmed by SDS-PAGE.
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Highest quality: high activity, specificity, thermostability, and performance in PCR.
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Highly efficient: reactivation buffer improved.
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Thermostable: half-life at 94 ºC is 40 minutes.
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Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
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Incorporates modified nucleotides: biotinylated, fluorescently labeled,
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Molecular Weight: 94 kDa.
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Convenient: available in different concentrations, sizes, and solutions.
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Complete solution: includes MgCl2.
Overview
Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
Storage
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Shipped in: Gel pack.
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Storage: -20 ºC (NON Frost-Free Freezer).
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Shelf Life: 24 months from the date of purchase, if it is properly stored.
Appllications
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Routine amplifications.
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Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
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Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
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PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).
For 500 U
100 μL Taq Polymerase (5 U/μL)
25 mM MgCl2 (1.5 mL)
1.5 mL Buffer (10x)
Content
Related Products
-
Red Ready Taq MasterMix
HotRun DNA Polymerase
Features
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High specificity: minimizes unspecific amplification.
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Efficient: prevents or minimizes primer-dimer and nonspecific products.
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Great sensitivity: amplifies from femptograms of DNA targets.
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Inactive: at Room Temperature.
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Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
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Powerful: amplification of targets up to 5 kb.
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Convenient: available in kits and MasterMix solution.
Overview
HotRun DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Appllications
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qPCR.
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PCR and RT-PCR.
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Genotyping with Taqman Probes.
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PCR fragments amplification for TA or GC cloning.
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Amplification from a limited DNA template or low copy number genes.
Content
for 500 U
100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL)
25 mM MgCl2 (1.5 mL)
1.5 mL Buffer (10x)
Storage
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Shipped in: Gel pack.
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Storage: -20 °C.
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Shelf Life: 24 months from the date of purchase, if it is properly stored.
Related Products
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Red Ready VIP Taq MasterMix
LongRun DNA Polymerase
Features
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High Fidelity: 6.5 times higher fidelity than Taq DNA Polymerase alone.
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Robust: PCR amplification of fragments up to 15 kb such as 5-15 kb of genomic DNA.
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High Yield: increased amplicon specificity and yield.
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Accurate.
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Versatile: proven performance for long or difficult templates.
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Risk-free: product covered by our Quality 100% Guarantee.
Overview
LongRun DNA Polymerase is an accurate and robust enzyme that combines Horse-Power™ Taq DNA Polymerase and a DNA proofreading Polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long range PCR).. As it is already well-known, Taq DNA Polymerase is inefficient at amplifying fragments larger than 3–5 kb due to its inability to repair nucleotide mismatches following misincorporation. The addition of a small quantity of proofreading enzyme allows mismatches to be repaired and extension to continue, resulting in the amplification of long amplicons with high yield. The presence of the proofreading polymerase significantly increases fidelity (6.5x) as compared to Taq DNA Polymerase alone. This mixture of enzymes allows for long and accurate PCR amplification of targets from a variety of templates, such as 5-15 kb of genomic DNA. It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. As well, it possesses 3´→ 5´ exonuclease activity and it generates PCR products with blunt ends and generate 3´-adenine overhang in amplified DNA and thus such Taq amplified DNA could be cloned into T-vectors.
Appllications
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PCR-Cloning.
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Primers extension.
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Long or difficult amplification.
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High-Throughput PCR.
Content
For 200U:
200 U FastPANGEA™ Long PCR DNA Polymerase (5 U/μl)
1.5 mL Buffer PA (10x)
1.5 mL MgCl2 (25 mM)
50 μL DMSO (100%)
Storage
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Shipped in: Gel pack.
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Storage: -20 °C.
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Shelf Life: 24 months from the date of purchase, if it is properly stored.
Related Products
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Red Ready VIP Taq MasterMix
Other DNA Polymerases
T4 DNA Polymerase
SNP Taq DNA Polymerase
BstTaq DNA Polymerase- No exonuclease
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Generation of blunt double-stranded DNA from DNA containing 5′ overhangs.
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Generation of blunt double-stranded DNA from DNA containing 3′ overhangs.
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5′-end or 3′-end labeling of double-stranded DNA.
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In vitro mutagenesis.
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High specific or Multiplex PCR.
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Real-Time PCR with intercalation dyes.
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High Fidelity dNTPs and ddNTPs.
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Mini Sequencing procedures.
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Allele-specific primer extension (AS-PEX).
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SNP genotyping by allele-specific PCR (AS-PCR).
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Single Nucleotide Polymorphism (SNP).
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Nucleic acid amplification methods.
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Whole genome amplification.
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Multiple displacement amplification.
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DNA Sequencing.