High purity enzymes and buffers. Optimized for the highest activiz
Enzymes
RNase A
Features
-
Small single-chain polypeptide: 124 residues, ~13.7 kDa.
-
Avoids the use of co-factors and divalent cations.
-
High specific activity: more than 100 Kunitz units per mg protein.
-
High Quality: free of DNase, Proteinase or exonuclease contamination.
-
Time-saving protocol: avoids the RNAse heat step before use.
-
Risk-free: product covered by our Quality 100% Guarantee.
Overview
RNase A (Ribonuclease A) is a bovine pancreatic endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2’, 3’-cyclic phosphate, which is then hydrolysed to the corresponding 3’-nucleoside phosphate.
Appllications
-
RNA removal during DNA isolation.
-
RNA sequence analysis.
-
RNase protection assays.
-
RNA quantification or mapping.
-
Purifying plasmid DNA.
-
Genomic DNA isolation.
-
Molecular weight marker.
-
1 mL RNase A Solution (10 mg/mL)
Content
Storage
-
Shipped in: Gel pack.
-
Storage: RNase A solutions retain enzymatic activity at room temperature or 2-8 ºC for extended periods. However store at –20 ºC in a freezer without a defrost cycle is recommended. Avoid freeze/thaw cycles.
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
Related Products
-
cDNA Synthesis kit
DNase I
Features
Features
-
Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer).
-
High specific activity: more than 2,000 Kunitz units per mg protein.
-
High Quality: free of RNase and Proteinase contamination.
-
Complete solution: supplied with 10x Reaction Buffer.
-
Risk-free: product covered by our Quality 100% Guarantee.
Overview
DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends.
It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2⁺ and Ca2⁺) for maximal activity.
Appllications
Content
-
Removal of residual genomic DNA from RNA samples.
-
Degradation of DNA template in transcription reactions.
-
DNAse I footprinting.
-
Perform Nick Translation.
-
1 mL DNase I (10 mg/mL)
-
1 mL Reaction Buffer (10x)
Storage
-
Shipped in: Gel pack.
-
Storage: -20 °C.
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
Related Products
-
cDNA Synthesis kit
Proteinase K
Features
-
Highly active and stable: > 30 units/mg protein (hemoglobin, pH 7.5, 37 °C).
-
High Quality: free of RNases, DNases and Exonucleases contamination.
-
Purified by chromatography.
-
Lyophilised format.
-
Low cutting specificity.
Overview
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight 28.900 kD, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. It is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).
Appllications
-
Isolation of genomic DNA from cultured cells and tissues.
-
Removal of DNases and RNases during DNA and/or RNA purification.
-
Determination of enzyme locations.
Storage
-
Shipment at Ambient Temperature.
-
Storage: 4 °C or -20 °C.
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
Content
-
Proteinase K (Lyophilized powder)
Related Products
-
cDNA Synthesis kit