High purity enzymes and buffers. Optimized for the highest activiz
Enzymes
T4 DNA Ligase
Features
-
Recombinant: isolated from E. coli.
-
Highly efficient: 3,500 white colonies and 10 blue colonies expected under optimal conditions.
-
Fast and easy protocol: get 24 % of efficiency at 5 minutes and 75 % at 15 minutes.
-
Powerful: allows cloning insert amounts < 1 ng/kb.
-
Versatile: enables sticky end or blunt end DNA Ligation.
Overview
T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.
Appllications
-
Routine cloning procedure: blunt and cohesive double stranded DNA cloning.
-
TA cloning.
-
Joining of adaptors with blunt or cohesive ends.
Storage
60 µL T4 DNA Ligase (5 U Weiss/µL)
250 µL T4 DNA Ligase Buffer (5x)
Content
Related Products
-
Shipped in: Gel pack.
-
Storage: -20 °C.
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
-
cDNA Synthesis kit
Exonuclease
Features
-
High Specific Activity: 185,000 U/mg.
-
3′ -> 5′ single strand exonuclease.
-
Versatile: suitable with a wide-range of Buffer conditions.
-
Compatible with Magnesium.
-
Complete solution: supplied with 10x Exonuclease I Reaction Buffer.
-
Risk-free: product covered by our Quality 100% Guarantee.
Overview
Exonuclease I, the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3′→5′ direction releasing 5′-mononucleotides and leaving the terminal 5′-dinucleotide intact.
Appllications
-
Removal of residual ssDNA, including oligonucleotides, from reaction mixes.
Storage
-
Shipped in: Gel pack.
-
Storage: -20 °C.
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
Content
-
For 5,000 U:
-
5,000 U Exonuclease I (20 U/μL)
-
375 μL Exonuclease I Reaction Buffer (10x)
-
10x Reaction Buffer: 670 mM Glycine-KOH, 67 mM MgCl2, 100 mM 2-mercaptoethanol (pH 9.5, 25 °C).
Related Products
-
cDNA Synthesis kit