RedReady Taq Polymerase
MasterMixes
HIGHLIGHTS RedReady Taq
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Engineered Taq combined with the advanced buffer that outperforms classical Taq Polymerase
- Premixed with red dye and density reagents for direct loading onto the gels after the PCR run
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High yields under standard and fast cycling
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Increased success in amplification of longer templates (6 kb)
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Robust amplification of GC/AT rich templates
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50µl final reaction volum
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Enigineered Hot-start Taq Polymerase with advanced buffer outperforming classical Hot-satrt Taq polymerases
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Works efficiently with Crude samples
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ideal for Low copy target DNA templates
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Amplification of complex (GC/AT rich) templates
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Fast PCR
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TA cloning
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Multiplex hot-start PCR
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50µl final reaction volumn
HIGHLIGHTS RedReady VIP-Taq
APPLICATIONS RedReady Taq
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Routine PCR up to 6 kb with a direct gel loading option
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Colony PCR
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TA cloning
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Amplification of complex (GC/AT rich) templates
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Fast PCR
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Hot-start long range PCR with a direct gel loading option
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PCR from crude samples
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Low copy target detection
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TA cloning
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Amplification of complex (GC/AT rich) templates
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Fast PCR
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Multiplex hot-start PCR
APPLICATIONS RedReady VIP-Taq
DESCRIPTIONS
ReadReady Taq DNA Polymerase MasterMix is designed for convenience and optimized to be consistent with various samples and protocols. The MasterMix contains a versatile engineered Taq DNA polymerase enzyme in an optimized buffer containing all necessary components for ideal PCR reactions. The accuracy of ReadReady Taq DNA Polymerase is about 4.5 x 104 (a number of correct nucleotides incorporated before the first error) and produces A-tailed products suitable for ligating into TA cloning vectors.
The Mastermix is provided as a 2X reagent providing not just convenience but also the additional advantage of reducing pipetting and handling errors minimizations.
ReadReady Taq DNA Polymerase MasterMIx is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.
The Mastermix supplied with PCR water, and the only thing to add is the template with primers.
ReadReady VIP-Taq DNA Polymerase MasterMix is a Hot-start technology with small molecular inhibition that is advantageous over normal Hot-start polymerases. Combined with a highly optimized buffer, the Mastermix offers excellent efficiency for demanding PCR applications like an amplification of complex templates and crude sample PCR. RedReady VIP-Taq DNA Polymerase Mastermix is engineered to be excellent for long run PCR, fast cycling PCR and TA cloning.
The Mastermix is provided as a 2X reagent providing not just convenience but also the additional advantage of reducing pipetting and handling errors minimizations.
ReadReady Taq DNA Polymerase MasterMIx is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.
The Mastermix supplied with PCR water, and the only thing to add is the template with primers.
PROTOCOL
For both ReadReady Taq and RedReady VIP-Taq Taq Polymerase Mastermixes
Tips for successful workflow
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Take typical measures to prevent PCR cross-over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
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Include a no-template control and positive control in parallel.
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Thaw and keep reagents on ice. Mix well before use.
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Use 15 sec/kb extension. The longer the amplicon, the longer the extension time
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Use 90-sec extension for multiplexing.
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For primer annealing optimization, run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
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Do not use fast cycling for multiplexing.
To Prepare a 50 μl reaction, mix the following:
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Reverse Primer: 100 - 400 nM final concentration
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Forward Primer: 100 - 400 nM final concentratio
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Template:
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cDNA: <100 ng
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gDNA 1µg: 5-500 ng
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fill with PCR water 20 25µl
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RedReady Taq DNA Polymerase (2X): 25µl
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Mix gently. Place into the instrument
PCR Program
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Initial denaturation: 1 cycle: 95°C - 1-2 min
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Denaturation: 40 cycles: 95°C - 15 sec
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Annealing: 40 cycles: 60-65°C - 15 sec
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Extension 40 cycles: 72°C – 15 sec/kb
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Load probes on the agarose gel. The red loading dye is included in the mastermix.
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Store probes for short time on ice, for long at -20°C.