Accurate, Sensitive & Easy method for studying Gene Reporter Regulation and Function in transformed Cell lines
The follwing assays are available to measure cellular Cytotoxicity:
Firefly Luciferase Assay
Advantages & Features
Easy and Fast protocol: results within 14 minutes.
Sensitive and linear: correlation between luciferase gene expression and light output for transfection.
Ideal for high throughput assays.
Risk-free: product covered by our Quality 100% Guarantee.
Detection and quantification of Firefly Luciferase.
Reporter enzyme from cultured cells.
Luciferase Assay Kit (from the firefly Photinus pyralis) is an accurate, sensitive and easy method for studying gene reporter regulation and function in transformed cell lines in culture.
Firefly Luciferase has an apparent molecular weight of 62 kDa, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes the oxidation of reduced luciferin in the presence of ATP-Mg2+ and oxygen to generate CO2, AMP, PPi, oxyluciferin and produces a flash of light that is proportional to the quantity of luciferase in the reaction mixture.
The Luciferase Assay Substrate includes coenzyme A, ATP and luciferin. Including coenzyme A in the reaction enhances the sensitivity of the assay and provides a sustained light reaction (half-life > 5 minutes). This eliminates the need for automated luminometer injection of substrate and allows analysis by photographic film or scintillation counting.
10 mL Luciferase Assay Substrate
4 mL Cell Lysis Buffer (5x)
10 μg Luciferase (Control)