qPCR
MasterMixes
HIGHLIGHTS
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Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions.
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ROX content option for best normalization calculations in different concentration to fit for almost all type of instruments.
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Works with high GC & high AT-rich templates
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Rapid extension, early Ct detections
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Very high sensitivity
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Minimum or no optimization is required
APPLICATIONS
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Relative gene expression analysis
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Absolute quantification of any RNA template (mRNA, total RNA, viral RNA) and low copy number genes
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qRT-PCR assays based on CYBER Green or specific Probes: including TaqMan®, Molecular Beacons, Scorpions™ Probes
DESCRIPTIONS
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2X master mix for 500 or 1000 reactions per 20µl reaction volume
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Supplied with PCR degree water
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To cover all RT-PCR instruments, the InView qRT-PCR MasterMixes are available in three versions – without ROX, with low or high ROX concentration. See the ROX concentration table below to know which ROX concentration suits your instrument
PROTOCOL
Tips for successful workflow