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Abstract Visual Art




  • Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions.

  • ROX content option for best normalization calculations  in different concentration to fit for almost all type of instruments. 

  • Works with high GC & high AT-rich templates

  • Rapid extension, early Ct detections

  • Very high sensitivity

  • Minimum or no optimization is required


  • Relative gene expression analysis

  • Absolute quantification of any RNA template (mRNA, total RNA, viral RNA) and low copy number genes

  • qRT-PCR assays based on CYBER Green or specific Probes: including TaqMan®, Molecular Beacons, Scorpions™ Probes



Tips for successful workflow

  • Use special primer selection programs for good planning.

  • Work with amplicons in a range of 80-200, max 400 bp.

  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.

  • Run reactions in triplets; include a no-template control, no RT Mix control and positive control in parallel.

  • Thaw and keep reagents on ice. Mix well before use.

  • Do not perform annealing/extension for more than 30 seconds and do not use lower than 60 °C temperature for this step.

To Prepare a 20 μl reaction, mix the following:

  • Reverse Primer: 100 - 400 nM final concentration

  • Forward Primer: 100 - 400 nM final concentration

  • Specific Probe 200 nM final c. (0.4 μl of 10 μM) "Only if Probe based MasterMix  is used. CYBER Green MasterMixes do not require Probes"

  • cDNA template 100 ng or gDNA 1µg

  • Add PCR Water to 10 μl volume

  • InView qRT-PCR MasterMix  (2X):  10 μl

  • Mix gently, avoid bubbles. Place into the instrument

    • If Probe based MasterMixe is used set the instrument channels according to the probes specifications

    • If CYBER Green MasterMix is used, set the instrument onto SYBR® Green or FAM channel

PCR Program

  • Initial denaturation 1 cycle: 95°C - 2 min for cDNA, or 1 cycle: 95°C - 3 min for gDNA

  • Denaturation 40 cycles: 95°C - 5 sec

  • Annealing/extension 40 cycles: 60-65°C – 20-30 sec


Question regarding orders or bulk orders, please contact us

For technical questions about the products please contact our customer support

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