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High purity enzymes and buffers. Optimized for the highest activiz


M-MLV reersetranscriptase

M-MLV Reverse Transcriptase


  • Concentration: 200 U/µL.

  • Full Solution includes Reaction Buffer 5x.

  • High Quality: free of DNAse, RNase or protease contamination.

  • Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.

  • Final Product Size: 7 kb or less.


  • RT PCR.

  • Synthesis of cDNA.

  • mRNA 5’-end Mapping by Primer Extension Analysis.

  • End-labeling of DNA.

  • Dideoxynucleotide Sequencing.


M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT. MW: 69 KDa.


  • Shipped in: Gel pack.

  • Storage: -20 ºC (NON Frost-Free Freezer).

  • Shelf Life: 24 months from the date of purchase, if it is properly stored.


  • M-MLV Reverse Transcriptase (200 U/μL)

  • Reaction Buffer (10x)


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