High purity enzymes and buffers. Optimized for the highest activiz
Enzymes
M-MLV Reverse Transcriptase
Features
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Concentration: 200 U/µL.
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Full Solution includes Reaction Buffer 5x.
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High Quality: free of DNAse, RNase or protease contamination.
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Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.
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Final Product Size: 7 kb or less.
Appllications
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RT PCR.
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Synthesis of cDNA.
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mRNA 5’-end Mapping by Primer Extension Analysis.
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End-labeling of DNA.
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Dideoxynucleotide Sequencing.
Overview
M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.
Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT. MW: 69 KDa.
Storage
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Shipped in: Gel pack.
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Storage: -20 ºC (NON Frost-Free Freezer).
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Shelf Life: 24 months from the date of purchase, if it is properly stored.
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M-MLV Reverse Transcriptase (200 U/μL)
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Reaction Buffer (10x)
Content
Related Products
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cDNA Synthesis kit
AMV Reverse Transcriptase
Features
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Concentration: 200 u/µL
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Full Solution: includes Reaction Buffer 5x.
-
High quality: free of RNase, and DNase (exo- and endonuclease) activities.
Appllications
-
RT PCR.
-
Synthesis of cDNA.
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RNA Sequencing.
Overview
AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus (AMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template.
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. The enzyme possesses an intrinsic RNase H activity. AMV RT possesses multiple enzymatic activities including RNA- and DNA-directed DNA polymerase, DNA-RNA unwinding activity, a sequence- specific Mn2+ -dependent endonuclease and ribonuclease H.
Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of AMLV-RT.
Storage
-
Shipped in: Gel pack.
-
Storage: -20 ºC (NON Frost-Free Freezer).
-
Shelf Life: 24 months from the date of purchase, if it is properly stored.
Content
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AMV Reverse Transcriptase (10 U/μL)
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Reaction Buffer 5x
Related Products
-
cDNA Synthesis kit